The Definitive Guide to principle of HPLC working
Analyte molecules partition among a liquid stationary period as well as the eluent. Just as in hydrophilic interaction chromatography (HILIC; a sub-method inside HPLC), this technique separates analytes determined by discrepancies of their polarity. HILIC most often uses a bonded polar stationary section plus a cell stage made mainly of acetonitril